Efficacy against footrot of a Bacteroides nodosus 265 (serogroup H) pilus vaccine expressed in Pseudomonas aeruginosa
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منابع مشابه
Efficacy against footrot of a Bacteroides nodosus 265 (serogroup H) pilus vaccine expressed in Pseudomonas aeruginosa.
Pili of Bacteroides nodosus 265 (serogroup H) were expressed in Pseudomonas aeruginosa both alone and in combination with pili from B. nodosus 198 (serogroup A). Pilin genes from B. nodosus were introduced on plasmid-borne, thermoregulated expression systems, either singly or as tandem genes from two separate strains in a single transcription unit. Despite the absence of a posttranslational cle...
متن کاملPilins from the B serogroup of Bacteroides nodosus: characterization, expression, and cross-protection.
Sequences of pilin genes from four strains of serogroup B of the ovine pathogen Bacteroides nodosus have been determined. These sequences permit comparisons of amino acid sequence between pilins from different subtypes (B1, B2, B3, B4) of the B serogroup and assessment of intraserogroup variation. Pili of B. nodosus strains 234 (B1) and 183 (B2) were produced by Pseudomonas aeruginosa harboring...
متن کاملMorphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa.
Type 4 fimbriae are found in a range of pathogenic bacteria, including Bacteroides nodosus, Moraxella bovis, Neisseria gonorrhoeae, and Pseudomonas aeruginosa. The structural subunits of these fimbriae all contain a highly conserved hydrophobic amino-terminal sequence preceding a variable hydrophilic carboxy-terminal region. We show here that recombinant P. aeruginosa cells containing the B. no...
متن کاملPhylogenetic analysis of Dichelobacter nodosus serogroup-specific fimA gene from ovine footrot in Andhra Pradesh
AIM Identification of different serogroups of Dichelobacter nodosus prevailing in the region and to understand the degree of genetic heterogeneities among the different isolates of D. nodosus. MATERIALS AND METHODS A total of 150 exudate samples of footrot lesions with a lesion score of 2-4 were collected from naturally infected sheep. The samples were screened by polymerase chain reaction (P...
متن کاملNucleotide sequence of the gene encoding the two-subunit pilin of Bacteroides nodosus 265.
The nucleotide sequence of the gene encoding pilin from Bacteroides nodosus 265 has been determined. The pilin is encoded by a single-copy gene, from which can be predicted a prepilin comprising a single protein chain of Mr 16,637. The prepilin sequence differs in several respects from the mature protein sequence. Seven additional N-terminal amino acid residues are present in prepilin, whereas ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1988
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.56.3.595-600.1988